Next Generation Sequencing

Whole genome sequencing (WGS) is the process of sequencing the entire DNA content of a microbial isolate. It is commonly used to identify and characterize isolated microorganisms. 

The gold standard for DNA fingerprinting of bacteria, yeasts, and molds, this technology offers the highest resolution of discriminatory power and can discern the relatedness of strains at the nucleic acid level.  

Mérieux NutriSciences has expertise in conducting Whole Genome Sequencing and providing detailed explanation of results for: 

• Strain Comparison
• Outbreak investigations
• Surveillance
• Source tracking for contamination events

All data is securely stored on a local server, and a dedicated library is prepared for your company so you can rest easy knowing your WGS data is protected. 

Also known as targeted metagenomics, DNA metabarcoding allows for massive genetic marker sequencing that provides taxonomic information in a sample. Mixed microbial communities in complex samples are sequenced to identify and determine the relative abundance of bacteria, yeast, and/or mold in the sample. 

Mérieux NutriSciences developed and uses its own continuously updated database with a robust bioinformatics pipeline for data analysis. Microbial metabarcoding is frequently used for:

• Surveying microbial communities
• Spoilage investigations
  • Shelf life testing
• Profiling organisms used in culturing and fermentation of products
• Environmental Monitoring

Evaluate the relative population of a mixed genomic community in a single analysis. Shotgun metagenomics repeatedly and randomly sequences all of the DNA fragments in a sample, whether bacterial, fungal, viral, plant, animal, etc. 

• Sequence directly from food sample to capture all DNA present
• Identify organisms to the species level
• Use as a tool in spoilage investigations
• Capable of identifying organisms to the species level

Mérieux NutriSciences tailors data analysis to your objectives and provides expert reporting to meet your needs.